Beilstein J. Org. Chem.2018,14, 1946–1955, doi:10.3762/bjoc.14.169
, Koganei, Tokyo 184-8588, Japan 10.3762/bjoc.14.169 Abstract Artificial orthogonal bond formations such as the alkyne–azide cycloaddition have enabled selective bioconjugations under mild conditions, yet naturallyoccurringlinkages between native functional groups would be more straightforward to
directly conjugated. It should be noted that there is no need to pre-install artificial functional groups and undesired and unpredictable perturbations possibly caused by bioconjugation can be minimized.
Keywords: alkyl chain soluble supports; artificial bioconjugates; naturallyoccurringlinkages; 5
linkage. Described herein is a simple and straightforward access to artificial bioconjugates with naturallyoccurringlinkages.
Results and Discussion
The present work began with the optimization of the reaction conditions for the activation of the 5’-terminus, more specifically, 5’-phosphitylation [74
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Graphical Abstract
Figure 1:
Schematic illustration of possible support-assisted methods.